Jim Fitzpatrick, food, farming and environment minister, left a ceremony at London Muslim Centre, Whitechapel.
The MP for Poplar and Canning Town told the BBC the segregation showed a degree of intolerance in the East End.
But a spokesman for the centre said the segregation was at the request of the couple getting married.
Mr Fitzpatrick, whose constituency is home to a large Muslim community, blamed the tough stance on the Islamic Forum of Europe (IFE) - an organisation that calls for Sharia law - which is based in the same building.
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